Antimicrobial activity in vitro of sunflower oil ozonated on Streptococcus mutans

Abstract

Streptococcus mutans is considered the main ethiological agent of
buccal caries. The aim of this work was to measure the lethality action of ozonized
sunflower oil (OLEOZON®) on this microorganism. The influence of different
conditions, like pH, agent concentration and contact time was evaluated. The
values of minimum inhibitory concentration (CMI) were determined. The CMI
shows similar behaviour among species of the same genus and different results
for bacterias from other gender. It was proved that OLEOZON® activity did not
vary significantly with the studied variation of pH, although the microorganism
number decreases to pH lightly acid the highest decreased of initial
microorganism concentration was about 99.9 %. The study of the influence of
the different agent concentrations showed a significant effect of OLEOZON® in
the microorganism lethality, being 285 mg/mL the most effective studied
concentration. A ten-minute exposition time, between the agent and the
microorganism, produces a 99.9 % of microorganism lethality; however with
only three minutes of exposition time a reduction of 95.5 % of initial
microorganism concentration was obtained. An equation which relates the study
of three main physico-chemical parameters was obtained. This equation shows
the influence of pH, antimicrobiological agent and exposition time upon of the
inactivation of the microorganism. These results proved that OLEOZON® is an
effective drug to treat Streptococcus mutans infection.