Establecimiento de la técnica de Bandeo - C en clones silvestres del complejo Saccharum.

Abstract

Sugarcane is a complex hybrid of autopolyploid nature with small chromosomes (1 – 3 μm), which difficult the chromosome identification and characterization. Up to now, studies on genome structure and evolution have been mainly based on the distribution of chromosome numbers and unknow the use of chromosome banding techniques, which are very useful for individual chromosome identification and as cytogenetic markers. Giemsa C - banding technique was established in new wild clones of Erianthus genus, testing different concentrations of hydrolysis from 0,05 N HCl to 0,2 N and times from 0,5 min. to 2 min. Variants of time in Giemsa stain were evaluated from 15 min. to 1,5 hours. At last, suitable hydrolysis concentration and time were resulted of 0,1 N HCl for 1 min., as well as time of Giemsa stain of 1 hour. Centromeric, interstitial bands and combinations of centromeric with telomeric and telomeric with interstitial were observed. These bands allowed to perform genotype characterization based on banding pattern.