Evaluación in vitro de la nefrotoxicidad por Cisplatino en corteza renal de ratas

Abstract

The cis-diamminedichloro platinum II (CDDP) is a powerful anticancer drug. The precise mechanism of CDDP nephrotoxicity is far too
clear, however several works relate it to oxidative stress. To study the action of this drug directly on the kidney and relationship to the redox renal status, the following study in vitro was carried out. The model utilized was rat renal cortical slices. Two experimental group were created, the control and the one exposed to Cisplatin, in this last one was dissolved the CDDP (2 mmol/L). Both groups were incubated to 30, 60,120 y 240 min. Cytotoxicity enzymes marker (y-glutamiltransferase (y-GT), N-acetilglucosaminidase (NAG), aspartateaminetransferase (ASAT) y lactatedeshidrogenase (LDH)), glutathione levels, malonildialdehide levels and the activities antioxidants enzymes (superoxide dismutase, total peroxidase and catalase were determined. The results showed that the Cisplatin caused an injury in the renal cortex, fundamentally on the proximales tubules suggested by the cytotoxicity enzymes. In turn there was an early decreased glutathione level, total peroxidase and superoxide dismutase activities and increment to malonildialdehúdo level and catalase activity. All these results point to the fact that the Cisplatin promotes an alteration on redox renal status, which can begin the events involved in the nephrotoxicity induced by CDDP.