DERMAL AND OPHTHALMIC IRRITABILITY in vivo OF A COSMETIC FORMULATION WITH NATURAL ACTIVES

Abstract

Propolis is a waxy resin of viscous consistency obtained by Apis mellifera, which has several associated effects: antimicrobial, analgesic, anti-inflammatory, antioxidant and healing action. It has been incorporated in various formulations for human use.  Sericin is a globular protein obtained from the cocoons of the silkworm Bombyx mori and has applications in the cosmetic industry for its antioxidant properties. Objective: To determine the dermal and ophthalmic irritancy of a cosmetic cream containing sericin hydrolysate and propolis. Materials and Methods: Experimental batch of the cosmetic cream and 6 male rabbits of the Cenp: NZW line with body weight 1.8 kg, as established by the Organization for Economic Cooperation and Development (OECD 2012;2015), were used. The principles of Good Laboratory Practices and ethical principles to avoid animal suffering during experimentation were complied with. In the skin as in the ocular structures the effects were observed immediately after application at 1, 24, 48 and 72 hours. The Draize scale was used to evaluate the lesions. Results: The cosmetic formulation is neither dermal nor ophthalmic irritant; dermal and ophthalmic irritability index values equal to 0 were reached in the tested rabbits. Conclusions: The cosmetic cream with the natural active ingredients sericin hydrolysate and propolis meets the requirements for bodily use on potential consumers