Semi-noenzymatic preparation of DNA Streptococcus pyogenes and separation of restriction macro-fragments with SmaI by Pulsed Field Electrophoresis in minigels

Authors

  • Yaumara López-Carballo
  • Yainelis Garrido Nicot
  • Esperanza Niubó
  • Nelson Diaz
  • Ana M. Riverón
  • Lilia López-Canovas

Abstract

Methods of molecular subtyping of microorganisms are the basis of current epidemiological surveillance programs.1 They allow the identification of molecular subtypes of pathogenic microorganisms, which facilitates their control and the taking of measures to prevent their dissemination. Pulsed Field Electrophoresis (CPE) is the gold standard for bacterial molecular subtyping because it allows the analysis of the complete genome of bacteria in a single experiment.2 Through CPE it is possible to separate restriction macro-fragments in the form of a band pattern of the genomic DNA of bacteria.

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Published

2020-12-22

How to Cite

López-Carballo, Y. ., Garrido Nicot, Y. ., Niubó, E., Diaz, N. ., Riverón, A. M., & López-Canovas, L. . (2020). Semi-noenzymatic preparation of DNA Streptococcus pyogenes and separation of restriction macro-fragments with SmaI by Pulsed Field Electrophoresis in minigels. NATIONAL CENTER FOR SCIENTIFIC RESEARCH (CENIC) BIOLOGICAL SCIENCES JOURNAL, 40(2), 125-127. Retrieved from https://revista.cnic.edu.cu/index.php/RevBiol/article/view/760

Issue

Section

Short communications