Validación de un método espectrofotométrico para la determinación del contenido de aldehídos en el aceite de girasol ozonizado (OLEOZON®)

Abstract

Ozonized sunflower oil (OLEOZON®) has excellent germicidal properties (germicidal, fungicidal, virusidal, and antiparasitary) and it is very useful in the treatment of infectious diseases. The validation of a spectrophotometric method for the determination of aldehyde content in OLEOZON® is presented in this work. The method is based on the formation of 2,4- dinitrophenylhidrazones of aldehydes, followed by the measurement of the absorbance at 338 nm. The parameters of linearity, precision, accuracy, specificity, and sensitivity were evaluated. The method employed was linear (r = 0.999 y r2 = 0.998), precise (repeatability CV = 0.38 %, intermediate precision CV = 0.81 %), exact (recovery ³ 97.8 %) and sensitive (detection limit = 1.04 · 10-9 mol/L and quantitation limit = 1.28 . 10-9 mol/L) in the interval of aldehyde concentrations studied. The method employed was specific to aldehydes, because the different compounds, which are present in OLEOZON®, such as peroxides, carboxylic acids, ozonides and polymeric compounds, do not interfere in the aldehyde content determination by this method. The aldehyde content with regards to nonanal, in a representative sample of OLEOZON® was (1.21 ± 0.04) mmol/g.