MSPEP: PROGRAMA PARA LA SINTESIS MULTIPLE Y SIMULTANEA DE PEPTIDOS

Abstract

Synthetic peptide have great applicability at the present time for their usefulness in the development of vaccines, such as specific antigens in immunodiagnostic for the detection of antibodies, in the study of the structure and the activity of the different active areas of the virus, among other applications . In this work the synthesis of several peptides corresponding to HCV, HIV, and Trypanosoma cruzi were described. The peptide design was carried out by means of calculation programs and consultation in protein databases, and in order to help the multiple and simultaneous peptide synthesis in solid phase, a new software was designed and developed. This application is executed on Microsoft Windows 95' using the inherent facilities to this graphic platform and it includes new options that are not in previous systems which are executed on MS-DOS. All the synthesized peptides were evaluated in the corresponding ultramicroELISA assays, with positive samples characterized for each etiologic agent, being obtained a sensitivity in HCV between 77,7 and 100 %, in HIV of 53 and 100 %, in-Trypanosoma cruzi, it was of 100%. In all the cases a specificity of 100 % was obtained. The software developed is of a great utility in the peptide synthesis because it guarantees speed and reliability in the chemical calculations with a minimum of error. As it is executed on Windows platform, it enlarges the possibilities of being more accessible and comfortable to the user