Obtaining a mixture of proteins with activity antitumor from marine bacteria

Abstract

Substances of marine origin have been used by man for millions
of years. However, their isolation, chemical and pharmacological characterization,
as well as the clinical interest in them, has been studied for only five
decades. Cuba has gradually increased the researches related to the search
for compounds with antitumoral activity. A strain of marine origin with antitumoral
activity and wide possibilities for human health preservation was
isolated at the Applied Microbiological Department of Marine Bioproduct
Center. The objective of the present work was to determine the culture conditions
of Micrococcus varians at fermentator scale to separate and to purify the
mixture of compounds produced and to evaluate its possible antitumoral activity
against different wicked tumors. The purification of the mixture of compounds
with antitumoral activity produced by this strain was carried out by
precipitation with (NH4)2SO4 70 % and dialysis. The purity approach was corroborated
by HPLC, anionic exchange chromatography and electrophoresis
in polyacrylamide (8-25 %). The antitumoral activity of the mixture of compounds
showed a survival index of 77,8 % against sarcoma 180 and a 37,3 %
against sarcoma 37.