ISOLATION AND CHARACTERIZATION OF BACTERIA FROM ACCOMPANYING OIL WATER PROMISING FOR BIODESULPHURIZATION

Abstract

Biodesulfurization is the process in which microorganisms are used to oxidize reduced sulfur compounds and transform them into easily removable compounds. This process has a set of advantages: low investment costs and operating because it uses simple equipment and low reagent consumption, low power consumption to be able to operate at room temperature. However, the process is slow, requiring new microorganisms that reduce the residence times and make the process more competitive in industry.  In this context, the present work proposes to isolate and identify microorganisms of accompanying oil water, as well as to determine their degradation capacity from sulfur compounds of the oil industry. The accompanying oil water from the oilfields of western North Strip Cuba was used for isolating as a microbial source and performed in selective medium with sodium thiosulfate and dibutil sulfide as the only sulfur source, the optical density of the cultures was determinated to evaluate microbial growth and the concentration of sulfate as degradation product of sulfur. As a result, 3 isolates belonging to the genus Pseudomonas sp with the property to biodegrade sulfur compounds were obtained. The isolates were identified as Pseudomona Ny 3a, Pseudomona Ny 3b, Pseudomona Ny 2a; highlights the letter two strain in medium with sodium thiosulfate and dibutil sulfide as the sole source of sulfur, respectively